LOW TEMPERATURE CASCADE

Low Temperature Cascade

low temperature cascade

 

A cascade sandwich is what we called this that day, built using two AHP-300CP cold plates. The customer needed a fairly large mass cycle between two extremes. The mass and size of the load were limiting factors. The pictures and assembly look crude, and it was, but they were also effective.

It was determined that a final design based on standard components would be successful and it was.

The AHP-300CP series is now replaced by a newer and more efficient model the AHP-470CP series.

TISSUE DISSECTION

Tissue Dissection Application:

AHP-1200CAS used in tissue dissection

We used TECA’s AHP-1200CAS three stage cascaded thermoelectric cold plate system to facilitate dissection of frozen human postmortem brain slices for extraction of protein for Proteomics research. Because we were trying to select specific regions without damaging the rest of each small specimen, it was important for us to maintain the integrity of the specimen during dissection.

Working in a lab at normal room temperature, we found that a constant temperature of -18 °C was ideal for this work (-20 °C was too cold, and warmer than -18  °C made the tissue start to melt). It would have been impossible to maintain such a precise temperature outside of a Cryostat without the cold table. It was immensely easier to perform the dissection on the cold table, and of course also much easier to wipe down the cold table than to clean a Cryostat.

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