TISSUE DISSECTION

Tissue Dissection Application:

AHP-1200CAS used in tissue dissection

We used TECA’s AHP-1200CAS three stage cascaded thermoelectric cold plate system to facilitate dissection of frozen human postmortem brain slices for extraction of protein for Proteomics research. Because we were trying to select specific regions without damaging the rest of each small specimen, it was important for us to maintain the integrity of the specimen during dissection.

Working in a lab at normal room temperature, we found that a constant temperature of -18 °C was ideal for this work (-20 °C was too cold, and warmer than -18  °C made the tissue start to melt). It would have been impossible to maintain such a precise temperature outside of a Cryostat without the cold table. It was immensely easier to perform the dissection on the cold table, and of course also much easier to wipe down the cold table than to clean a Cryostat.

LIQUID CHILLER COLD PLATE CASCADE

Liquid Chiller Cold Plate Cascade:

Here are pictures of the TLC-900 thermoelectric liquid chiller with LHP-150CP Peltier liquid cooled cold plate set up run in the lab. The “load” is a plate with 10 power resistors mounted to the top and a series of thermocouples measuring the surface of the LHP-150CP’s thermoelectric cold plate.

liquid chiller cold plate cascade liquid chiller

 

During the test the power to the resistors is increased in steps with enough time in between for the temperatures to stabilize. Plotting the data resulted in performance curves. The test was made with the load insulated and with the load simply covered.

Later on we used a TLC-900CAS, a version of the TLC-900 made specifically for running and controlling secondary cold plates like the LHP-150CP and ran some step, ramp and soak profiles.

STIRRING COLD PLATE

Stirring Cold Plate:

A Peltier stirring cold plate was needed to cool liquid inside a standard 1000 ml plastic tank and maintain the class 100 clean room rating. They desired a custom designed thermoelectric stirring cold plate with integral variable stir speed control, temperature control, alarm and remote sense capability.

stirring cold plate stirring cold plate

TECA is glad to participate in custom and OEM product development. Over the years we have come up with some innovative and novel solutions for our customers. This product was developed from scratch for a start-up company in the field of pharmaceutical development. This TECA cold plate apparatus was designed and built for use in a clean room facility. The controls are ergonomic. The user can easily adjust controls for stir speed, timing and temperature even while wearing clean room garb and sterile gloves.

COOLING GAS FLOW

Cooling Gas FLow:

A potential customer expressed interest in a cooling/heating system for an aerosol flow tube envisioning a 1 inch OD, 0.875 inch ID stainless steel flow tube mounted inside a 2-2.5 inch ID pipe (likely also stainless steel and insulated on the outside) capable of controlling the tube at temperatures ranging from roughly 5 °C to 40 °C at an ambient temperature of 25-30 °C.  We are measuring the change in particulates in the air due to the temp change.

cooling gas flow cooling gas flow

Standard liquid chillers were first offered along with technical design assistance. The requirements led to direct contact cooling as being a good solution. Standard cold plate products and controllers were offered which led to this request for a custom package. “We would like to have the flow tube sandwiched between the cold plates with the temperature controller and power supplies attached in a similar manner so that we only need attach the temperature controller (the TC-3400 appears as if it will work) to a computer so that we can change the set points remotely.

Is any of this even possible or am I dreaming in Technicolor?” We advised him that the best dreams were in color. Preliminary design work was done and a quote prepared. Shortly thereafter an order was received; design documents were completed and sent to production. Less than 3 months after the initial contact the product was shipped. Several months later a follow up was made with this response.

“Things are doing really well. It does exactly what we wanted it to do, and is much more efficient and better than we hoped for. We’re very happy!

COOLING FLUID FLOW

Cooling Fluid Flow:

cooling fluid flow

A customer needed to cool high pressure flow of fluid in a single pass. Using expanded Aluminum foam and attaching it to the surface of AHP-301CP thermoelectric cold plate (peltier cold plate) provided a way to maximize the thermal contact surface and eliminated the need to complex fluid path and larger cold plate.

FLUID DISPENSING

Fluid Dispensing:

fluid dispensing

A leading manufacturer of fluid dispensing systems uses one of our Thermoelectric Cold Plate and Temperature Controller for controlling the fluid temperature of adhesives, sealants, lubricants and other materials in meter, mix and dispensing systems.
Unlike other larger, higher cost and less accurate heating and cooling devices, which use heater cartridges, refrigerant or water to heat and cool, these thermoelectric-effect temperature controllers use the Peltier-Seebeck effect to directly convert electrical voltage to thermal differentials. This effect, which is reversible, either heats or cools fluid materials with precision to the temperature set point. The compact Peltier thermoelectric device, temperature sensor and the temperature control is mounted to a manifold where the fluid passes through to be precisely heated, maintained or cooled.

AHP-800MSP STIRRING COLD/HOT PLATE

COLD WELL

Cold Well:

cold well cold well

The CWB-01 cold well was first developed as an AHP-1200CPV custom accessory for the petrochemical industry. It replaced the need to maintain ice baths for the storage of chemicals. It has the advantage of good temperature control in either heating or cooling modes. The temperature of the cold plate can be referenced to the fluid temperature or the remote sensibility feature of the CPV can be used to control based on the actual fluid temperatures.

Cold Wells can be made to accommodate many vessel sizes or the ICE-1200 accessory with TECAlab Thermal Beads works well also.

COOLING FLUID FLOW

Cooling Fluid Flow:

cooling fluid flow cooling fluid flow

Chilling liquids being circulated by a peristaltic pump under ultra-clean conditions. When handling and processing various fluids in laboratory and electronic environments cleanliness is a must. Peristaltic pumps have been developed for the purpose of creating a fluid flow without contacting the fluid itself. TECA developed a liquid chiller to cool fluids, while satisfying the same cleanliness requirements.

The solution, based on our successful cold plate product line, has proven to be versatile and effective. Pictured here is the first prototype of a multiple-pass tube cooler for use with a peristaltic pump. Applications range from laboratory to medical to industrial.

R & D LABORATORY

R & D Laboratory:

r & d laboratory
An R&D laboratory needed to quickly cool beakers of high-concentration sucrose samples to 10 °C.  The customer reports that using TECA’s AHP-800MSP Magnetic Stirring Cold/Hot Plate system has been successful.

The customer has different sizes of samples, which are accommodated by TECA’s sleeve accessories. The customer uses 2L, 1.5L, 1L and 800mL sleeves at different times with the AHP-800MSP system. The sleeves are easy to switch in and out and add versatility to the system.

In fact, unique to this product is the adjustable nature of many of its features: stir-bar speed, fan speed (for noise reduction), and temperature sensing (internal or external).

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